Jevan Medlock

Jevan Medlock1

PhD student

Jevan started his PhD project in Paunov Research Group at the Department of Chemistry of the University of Hull in October 2014.

PhD supervisors: Prof. Vesselin N. Paunov (PI) and Dr Leigh Madden (Co-PI)

Research project 1:

Bioimprinting technology for cell shape recognition

Biomprinting technology has been recently developed to capture proteins, viruses and entire living cells via their structural and chemical information. Here we report a novel method for fabrication of polydimethylsiloxane (PDMS) cell imprints which capture the cellular morphological structure and can be used for cell shape recognition. Our cell shape replicating technique can permanently capture an impression of biological samples into polymer surfaces with promising approaches for early cancer diagnosis [1], developing selective antimicrobial therapies and formulations in the pharmaceutical industry [2] and for use in biological studies. In this work, we introduce the imprinting technique to replicate Jurkat cells. Jurkat cells were inactivated with fixatives to preserve the structural and morphological information on the cell surface. Our method bypasses the inconvenience of imprinting live cells due to their fragile nature and deformability. After fixation, the cell monolayers were immobilised on a polyelectrolyte treated glass slides and partially protected by with glucose solution. Curable PDMS was used to the imprint the exposed part of the cell monolayer and was peeled off from the template slide after curing. We studied the ability of the produced PDMS cell imprints to recognise the original cells by incubating them with Jurkat cell suspensions. We have conducted cell recognition experiments as a function of the cell concentration and surface coating of the produced cell imprints. The results indicate that the cell imprinting technology can be used for separation of cells of different shape and morphology. We demonstrate the selectivity of the cell imprints in retention of the cells of matching shape in a mixture with other cells. This technology is expected to find application in cell separation devices.

Bioimprints

Fig. 1 SEM images of PDMS bioimprints of Jurkat cells.

References

  1. Sorting Inactivated Cells Using Cell-Imprinted Polymer Thin Films. Ren K, Banaei N, and Zare RN. ACS Nano, 2013, 7 (7), 6031-6036.
  2. Borovička JStoyanov SDPaunov V N. Shape recognition of microbial cells by colloidal cell imprints. Nanoscale 2013 5 (18), 8560-8568.